Twilight could refer to:
- Starships and vehicles:
- Twilight (starship), a Cheetah-class Lyran Star Empire frigate
- Locations
- Twilight Nebula sector, a sector near the Klingon border
- Twilight Base, a planet within the Twilight Nebula
- Twilight Nebula, a nebula that blocked subspace communications
- Twilight Nebula sector, a sector near the Klingon border
- Real world topics:
- Twilight (episode), an episode of Star Trek: Enterprise
- Twilight (novel), a Star Trek: Deep Space Nine novel
- Twilight's End, a TOS novel
- Twilight's Wrath, a short story in Tales of the Dominion War
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